PROVO, JAMES NATHAN, M.S. Impact of Trans-10, cis-12 Conjugated Linoleic Acid on Interleukin (IL)-6 and IL-8 and Adipogenic Genes in Cultures of Human Adipose

Previously, we reported that primary cultures of human stromal vascular (SV) cells containing newly differentiated adipocytes treated with 30 uM trans-10, cis-12 CLA for 24 h had greater gene expression and protein secretion of IL-6 and IL-8 than vehicle controls (Brown et al. 2004). In addition, cultures treated with 30 uM trans-10, cis-12 CLA had decreased mRNA levels of PPARγ2 and several of its downstream target genes. However, the effects of trans-10, cis-12 CLA treatment on human adipose tissue explants are unknown. Therefore, we examined the extent to which trans-10, cis-12 CLA increased IL-6 and IL-8 gene expression and protein secretion, and decreased mRNA levels of PPARγ2 and several of its downstream targets in human subcutaneous adipose tissue explants. Explants (~ 500 mg each) from human subcutaneous adipose tissue were treated with either vehicle (BSA) or 30 uM trans-10, cis-12 CLA (CLA) for 8, 24, or 72 h. Trans-10, cis-12 CLA treatment increased IL-6 and IL-8 secretion into the media (Main effect of treatment: p < 0.05). Evaluation of the treatment by time interactions revealed that cultures treated with trans-10, cis-12 CLA for 72 h had higher (p < 0.05) levels of IL-6 and IL-8 in the media compared to the BSA controls. Similarly, trans-10, cis-12 CLA treatment increased the mRNA levels of IL-6 and IL-8 (Main effect of